AGO4, Argonaute 4
| Catalog Number | Pack Size | List Price* | Quantity | |
|---|---|---|---|---|
| AS09 617 | 50 µg | 417,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Binds endogenous siRNAs, preference for 24nt siRNAs with 5' A.Note that the AGO4 antibody reacts with the NEB prestained protein marker.
| Category | Antibody |
| Supplier | Agrisera |
| Application | CoIP (coIP), ICC, IP, WB |
| Regulatory Status | RUO |
| Research Field | RNAi/siRNA/miRNA |
| Species Specificity | Plant |
| Immunogen | KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5 , TAIR: At2g27040 |
| Host | Rb |
| Crossreactivity | Plant |
| Clonality | Pab |
| Format | Lyophilized |
| Working Dilution | 1 : 100 (ICC), 5 g of antibody per 1 gram of a fresh tissue (IP),1 : 2000-1 : 5000 (WB) |
| Purity | Immunogen affinity purified serum in PBS pH 7.4. |
| Storage Conditions | Store lyophilized/reconstituted at -20°C; Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of th... |
| References | Antibodies cited in scientific publications |
| Tech. Data Sheet | View datasheet |
| Link To Supplier | https://www.agrisera.com |
| Material Safety Data Sheet | Download PDF |
