Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 405


Catalog Number Pack Size List Price* Quantity
A48275 1 mg 417,00 Add

* CHF, excl. 8.1% VAT and shipping costs


Product Description

To minimize cross-reactivity, the goat anti-human IgG whole antibodies have been cross-adsorbed against serum proteins from bovine, mouse, and rabbit. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staini

Category Antibody
Supplier ThermoFisher
Application Secondary antibody, ICC
Regulatory Status RUO
Immunogen Gamma Immunoglobins Heavy and Light chains
Host Gt
Crossreactivity Hu
Clonality Pab
Format Liquid; proprietary buffer; pH 6.5, with 0.016% Bromonitrodioxane, 0.016% Methylisothiazolone
Concentration 2 mg/mL
Working Dilution Immunocytochemistry (1-10 µg/mL)
Purity Affinity chromatography
Conjugation Alexa Fluor Plus 405
Storage Conditions 4° C, store in dark
Tech. Data Sheet View datasheet
Link To Supplier https://www.thermofisher.com
Material Safety Data Sheet Download PDF