Nedd8 Conjugation Kit
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
J3150-1KIT | 1 Kit | 610,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Please inquire a quote for two or more UBPBio products
Nedd8 Conjugation Kit is designed for assaying the conjugation of Nedd8 to protein substrates in vitro. In a protein neddylation reaction, Nedd8 is first activated by the Nedd8 activating enzyme APPBP1/UBA3 (E1) in an ATP hydrolysis-dependent manner; activated Nedd8 is then transferred to the Nedd8 conjugating enzyme Ube2M (UbcH12) (E2) by formation of a thiolester bond with the catalytic cysteine residue of UbcH12I; Nedd8-charged Ube2M (UbcH12) can mediate protein neddylation in the presence of an appropriate Nedd8 ligase (E3).
Category | Kit - molecular biology |
Supplier | UBPBio |
Regulatory Status | RUO |
Species Specificity | Hu |
Format | 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol |
Storage Conditions | -80°C |
Disclaimer | Shipped on dry ice - extra freight charge will occur. Attention: keep resins in all kits at 4 °C |
Tech. Data Sheet | View datasheet |
Link To Supplier | http://www.ubpbio.com |
Shipping Details | Dry ice |
Material Safety Data Sheet | Download PDF |