Sumo1, Sumo2, Sumo 3 Conjugation Kit


Catalog Number Pack Size List Price* Quantity
J3140-1KIT 1 Kit 723,00 Add

* CHF, excl. 8.1% VAT and shipping costs


Product Description

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Sumo1, Sumo2, Sumo 3 Conjugation Kit is designed for assaying the conjugation of Sumo1, Sumo2 and Sumo3 to protein substrates in vitro. In a protein sumoylation reaction, a Sumo protein is first activated by the Sumo activating enzyme SAE1/SAE2 (E1) in an ATP hydrolysis-dependent manner; activated Sumo is then transferred to the Sumo conjugating enzyme Ube2I (E2) by formation of a thiolester bond with the catalytic cysteine residue of Ube2I; Sumo-charged Ube2I can mediate protein sumoylation in the presence of an appropriate Sumo ligase (E3).

Category Kit - molecular biology
Supplier UBPBio
Regulatory Status RUO
Species Specificity Hu
Format 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
Storage Conditions -80°C
Disclaimer Shipped on dry ice - extra freight charge will occur. Attention: keep resins in all kits at 4 °C
Tech. Data Sheet View datasheet
Link To Supplier http://www.ubpbio.com
Shipping Details Dry ice
Material Safety Data Sheet Download PDF