Linear polyUb Chain-binding Protein Identification / Validation Kit
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
J2520-1KIT | 1 Kit | 610,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Please inquire a quote for two or more UBPBio products
This kit is designed for two purposes: 1) identification of proteins that specially bind linear polyUb chains in whole cell or tissue lysates; 2) validation of direct interactions between linear polyUb chains and their binding proteins. The non-cleavable 6xHis-tagged linear Ub6 is resistant of deubiquitination when incubating with whole cell or tissue lysates, thus allowing maximally capture proteins that specifically bind linear polyUb chains. After binding, 6XHis-tagged non-cleavable linear Ub6 and the binding proteins can be enriched with Nickel XPure Agarose Resin (included). The bound proteins and the non-cleavable polyUb chains can be eluted from Ni resin using a buffer containing 250 mM imidazole (included) or by incubating with TEV protease (not included). Either of the elution method can significantly reduce the amounts of non-specific binding proteins when compared with elution using SDS sample buffer.
Category | Kit - molecular biology |
Supplier | UBPBio |
Regulatory Status | RUO |
Species Specificity | Hu |
Format | 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol |
Storage Conditions | -80°C |
Disclaimer | Shipped on dry ice - extra freight charge will occur. Attention: keep resins in all kits at 4 °C |
Tech. Data Sheet | View datasheet |
Link To Supplier | http://www.ubpbio.com |
Shipping Details | Dry ice |
Material Safety Data Sheet | Download PDF |