Fluorescein labeled K63-Ub2
| Catalog Number | Pack Size | List Price* | Quantity | |
|---|---|---|---|---|
| D2110-25uG | 25 µg | 517,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Please inquire a quote for two or more UBPBio products
This product contains an authentic K63-linked isopeptide bond, synthesized by using an enzymatic reaction containing UbE1, UbE2N, Ub(K63R) and Ub-fluorescein. Fluorescein is labeled on the C-terminus of Ub by mutating glycine 76 to a cysteine. In fluorescein labeled K63 Ub2, fluorescein is labeled on the proximal Ub. It is an excellent tool for monitoring deubiquitination that can be visualized by in-gel fluorescent detection of the production of fluorescein labeled monoUb (excitation at ~490 nm, and see product data sheet for examples). The assay is more quantitative and sensitive than immunoblotting of Ub. The detection limit is 10 ng or less.
| Category | Protein |
| Supplier | UBPBio |
| Regulatory Status | RUO |
| Species Specificity | Hu |
| Format | 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol |
| Storage Conditions | -80°C |
| Disclaimer | Shipped on dry ice - extra freight charge will occur |
| Tech. Data Sheet | View datasheet |
| Link To Supplier | http://www.ubpbio.com |
| Shipping Details | Dry ice |
| Material Safety Data Sheet | Download PDF |
