Anti-PsbA, D1 protein of PSII, C-terminal (rabbit antibody) (thylakoid membrane marker)


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AS05 084 50 µl 423,00 338,40 Add

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Product Description

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Due to biology of PsbA (D1) protein a number of degradation products can apprear in a sample and may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24 kDa and 16 kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands.Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.This product can be sold containing ProClin if requested.

Category Antibody
Supplier Agrisera
Application IF, ImmunoGold (IG), WB
Regulatory Status RUO
Research Field Plant Biology, Photosynthesis
Species Specificity Plant
Immunogen KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populu...
Host Rb
Crossreactivity Plant
Clonality Pab
Format Lyophilized
Working Dilution 1: 500 (IF), 1: 200 (IG), 1 : 10 000 (WB)
Grade Serum
Storage Conditions Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them ...
References Antibodies cited in scientific publications
Tech. Data Sheet View datasheet
Link To Supplier https://www.agrisera.com
Material Safety Data Sheet Download PDF