RNA Labeling Kit – 555

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Product Description

The RNA Labeling Kit produces alkyne-labeled RNA by in vitro transcription from bacteriophage T7 RNA polymerase promoters. The kit utilizes 5-Ethynyl-UTP (5-EUTP) nucleotide to firstly decorate the RNA with alkynes during enzymatic sznthesis. The resulting RNA contains alkyne modified uracils, which subsequently react with azido-derivates of e.g. fluorescent dyes, haptenes and others in a highly selective fashion under benign click reaction conditions. One adevantage is that the label incorporation can be tuned by the amount of marker azide used in the click reaction.