Ubiquitin/SUMO/Nedd8 E2 Screening Kit


Catalog Number Pack Size List Price* Quantity
J1300-1KIT 1 kit 1'888,00 Add

* CHF, excl. 8.1% VAT and shipping costs


Product Description

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The E2 Screening Kit II (Cat# J1300) contains ubiquitin (Ub)-activating enzyme (UbE1), SUMO-activating enzyme (6xHis-SAE1/SAE2), Nedd8-activating enzyme (APPBP1/6xHis-UBA3), Ub, SUMO2, Nedd8, and 29 Ub-conjugating enzymes (E2s). It can be used for 1) identification of the E2 enzyme paired for a specific Ub ligase (E3) for a protein substrate, and 2) assessing/profiling E2 activity by monitoring the E2-Ub thioester conjugate formation under non-reducing condition. Comparing to the E2 Screening Kit (Cat# J1100), this kit contains additional SUMO or Nedd8-activating E1, SUMO2 and Nedd8, which allow to assess SUMOylation and neddylation.

Category Kit
Supplier UBPBio
Regulatory Status RUO
Format 20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
Storage Conditions -80°C
Disclaimer Shipped on dry ice - extra freight charge will occur
Tech. Data Sheet View datasheet
Link To Supplier http://www.ubpbio.com
Shipping Details Dry ice
Material Safety Data Sheet Download PDF