Phosphotyrosine Monoclonal Antibody (pY20), AP
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
037722 | 100 µg | 588,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This antibody is known to show reactivity towards phosphothreonine. The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, WB |
Regulatory Status | RUO |
Immunogen | Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine |
Host | Ms |
Clone Number | [pY20] |
Isotype | IgG2b |
Crossreactivity | Chemical |
Clonality | Mab |
Format | Liquid; TBS; , with 1% BSA, 1mM MgCl2, 40% glycerol and 0.1% sodium azide |
Concentration | 1 mg/mL |
Working Dilution | Western Blot (1:1,000), ELISA (Assay-dependent) |
Purity | purified |
Conjugation | AP |
Storage Conditions | -20°C |
Disclaimer | Alternative CatNo: 03-7722 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |