CD3e Monoclonal Antibody (145-2C11), FITC, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
11-0031-86 | 1 mg | 590,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells. Applications Reported: This 145-2C11 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 145-2C11 antibody has been tested by immunohistochemistry on frozen mouse spleen and can be used at less than
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, ICC, IHC |
Regulatory Status | RUO |
Host | Hm |
Clone Number | [145-2C11] |
Genebank | CD247 |
Isotype | IgG |
Crossreactivity | Ms |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.5 mg/mL |
Working Dilution | Flow Cytometry (0.5 µg/test), Immunocytochemistry (10 µg/mL), Immunohistochemistry (Frozen) (10 µ... |
Purity | Affinity chromatography |
Conjugation | FITC |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 11003186 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |