Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

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Product Description

Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin. Applications Reported: This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis. Applications Tested: The FITC conjugated rat IgG2a isotype control is offered in 2 formats: - µg size: This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells and can be used at 5 µL for the catalog number ending in -41 or -42 and 20µL/test for the product ending in -73 per test. Each test size contains 0.5 µg. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls