Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

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Product Description

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control. Applications Reported: FITC Mouse IgG1 K Isotype Control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis. Applications Tested: This Mouse IgG1 K Isotype Control is offered in 2 formats: - µg size: This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they h