Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™

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Product Description

Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies. Applications Reported: This mouse IgG2a isotype control has been reported for use in cell surface and intracellular flow cytometric analysis, immunocytochemistry, and immunohistochemistry. Applications Tested: The FITC conjugated mouse IgG2a isotype control is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and should be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at test size: 5 µL 1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-s