Mouse IgM Isotype Control (11E10), FITC, eBioscience™

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Product Description

Description: The mouse IgM is useful as an isotype control immunoglobulin. The immunoglobulin was generated by immunization with lipopolysaccharide (LPS). The immunoglobulin is reactive with LPS. Applications Reported: This mouse IgM isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis. Applications Tested: This mouse IgM isotype control has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes and can be used at the same concnetration as the experimental antibody. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antib