CD25 Monoclonal Antibody (CD25-4E3), PE, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
12-0257-42 | 100 Tests | 304,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: This CD25-4E3 monoclonal antibody reacts to human CD25, which is also known as the low-affinity interleukin (IL)-2 receptor alpha. CD25 is expressed on activated T cells (including a subset of regulatory T cells), B cells, and macrophages. This receptor subunit associates with CD122, the IL-2 receptor beta chain, and CD132, the common gamma chain, to form the high-affinity IL-2 receptor. IL-2 receptor signaling has been linked to cell proliferation and survival in lymphocytes. CD25 is a marker for Hodgkin's disease, and has been associated with such autoimmune diseases as Type I diabetes. This monoclonal antibody is cross-reactive to rhesus monkey. Applications Reported: This CD25-4E3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This CD25-4E3 antibody has been pre-titrated and tested by flow cytometric analysis of normal peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturin
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Ms |
Clone Number | [CD25-4E3] |
Genebank | IL2RA |
Isotype | IgG2b,k |
Crossreactivity | Hu, Primate |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.2% BSA and 0.09% sodium azide |
Concentration | 5 µL/Test |
Working Dilution | Flow Cytometry (5 µL (0.125 µg)/test) |
Purity | Affinity chromatography |
Conjugation | PE |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 12025742 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |