FOXP3 Monoclonal Antibody (FJK-16s), PE, eBioscience™

Catalog Number Pack Size List Price* Quantity
12-5773-82 100 µg 540,00 Add

* CHF, excl. 8.1% VAT and shipping costs

Product Description

Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human. Please note that FJK-16s has been optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (cat. 00-5523). The use of other fixation and staining buffers is not recommended. Applications Reported: This FJK-1

Category Antibody
Supplier ThermoFisher
Application FC
Regulatory Status RUO
Host Rt
Clone Number [FJK-16s]
Genebank FOXP3
Isotype IgG2a,k
Crossreactivity Bo, Dg, Ca, Ms, Pg, Rt
Clonality Mab
Format Liquid; PBS; pH 7.2, with 0.09% sodium azide
Concentration 0.2 mg/mL
Working Dilution Flow Cytometry (1 µg/test)
Purity Affinity chromatography
Conjugation PE
Storage Conditions 4° C, store in dark, DO NOT FREEZE!
Disclaimer Alternative CatNo: 12577382
Tech. Data Sheet View datasheet
Link To Supplier
Material Safety Data Sheet Download PDF