TCR V alpha 2 Monoclonal Antibody (B20.1), Biotin, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
13-5812-85 | 500 µg | 458,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. This antibody recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b and c haplotypes. The antibody also reacts with the products of T cell receptor, V delta 8 due to the high degree of homology. Applications Reported: This B20.1 antibody has been reported for use in flow cytometric analysis and immunoprecipitation. Applications Tested: This B20.1 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended to preblock Fc receptors for 10 minutes using 0.5 µg of purified anti-mouse CD16/32 (cat. 14-0161). It is also recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptiv
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, IP |
Regulatory Status | RUO |
Host | Rt |
Clone Number | [B20.1] |
Isotype | IgG2a,l |
Crossreactivity | Ms |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.5 mg/mL |
Working Dilution | Flow Cytometry (0.125 µg/test), Immunoprecipitation (Assay-Dependent) |
Purity | Affinity chromatography |
Conjugation | Biotin |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 13581285 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |