CD4 Monoclonal Antibody (RM4-5), eBioscience™

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Product Description

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5. Applications Reported: The RM4-5 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. It has also been reported in isolation and depletion of CD4+ cells and blocking of ligand binding. (Please use Functional Grade purified RM4-5, cat. 16-0042, in functional assays.). Applications Tested: The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal pe