CD27 Monoclonal Antibody (LG.7F9), eBioscience™

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Product Description

Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27. Applications Reported: The LG.7F9 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. Use of mild detergent lysates is recommended when performing immunoprecipitation with this mAb. LG.7F9 has also been reported in blocking of ligand binding. (Please use Functional Grade purified LG.7F9, cat. 16-0271, in functional assays.). Applications Tested: The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determ