CD40 Monoclonal Antibody (HM40-3), eBioscience™

* CHF, excl. 8.1% VAT and shipping costs

Product Description

Description: The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. Applications Reported: The HM40-3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. HM40-3 has also been reported in inhibition of binding of soluble CD154 to CD40 and in vitro activation of B cells. (Please use Functional Grade purified HM40-3, cat. 16-0402, in functional assays.). Applications Tested: The HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined