Armenian Hamster IgG Isotype Control (eBio299Arm), eBioscience™

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Product Description

Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry. Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood. Use isotype control at the same concentration as experimental antibody. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP)