IL-1 beta Monoclonal Antibody (B122), eBioscience™

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Product Description

Description: The B122 antibody reacts with mouse and rat interleukin-1beta (IL-1beta), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. Applications Reported: The B122 antibody has been reported for use in cytokine neutralization, immunoblotting (WB), and ELISA. Please refer to Functional Grade purified B122 (cat. 16-7012) for functional assays. Applications Tested: The B122 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 beta (IL-1 beta) in combination with the biotinylated polyclonal anti-mouse interleukin-1 beta for detection and recombinant mouse IL-1 beta (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthe