TNF alpha Polyclonal Antibody, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
14-7341-85 | 500 µg | 328,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The affinity purified polyclonal rabbit antibody reacts with mouse and rat tumor necrosis factor-alpha. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4 T cells, and NK cells. A 26 kDa form is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Bioactive TNF alpha exists as a homotrimer. Applications Reported: This affinity purified anti-mouse/rat TNF alpha antibody has been reported for use as the plate-coating (ELISA Capture) antibody for the In Vivo Cytokine Capture Assay (IVCCA), in conjunction with the Functional Grade Biotinylated TN3.19 antibody for In Vivo Capture (ELISA Detection). The IVCCA facilitates measurement of cytokines in serum by increasing their in vivo half lives dramatically. This increases the sensitivity of measurement of in vivo cytokine production 30- to 1000- fold. For detailed methodology in this application, please refer to this reference: Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, Ne
Category | Antibody |
Supplier | ThermoFisher |
Application | FA |
Regulatory Status | RUO |
Host | Rb |
Genebank | TNF |
Isotype | IgG |
Crossreactivity | Ms, Rt |
Clonality | Pab |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.5 mg/mL |
Working Dilution | Functional Assay (Assay-Dependent) |
Purity | Affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | 4° C |
Disclaimer | Alternative CatNo: 14734185 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |