TNF alpha Monoclonal Antibody (TN3-19.12), eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
14-7423-85 | 500 µg | 333,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor-alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed. Applications Reported: The TN3-19.12 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, ELISPOT, IVCCA, and ELISA. Fluorochrome conjugated TN3-19.12 is recommended for use in intracellular staining and flow cytometry. Functional Grade purified TN3-19.12, cat. 16-7423, is recommended for use in functional assays. For ELISPOT capture, the alternative clone, 1F3F3D4 (cat # 16-7325), is recommended. Applications Tested: The TN3-19.12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse or rat TNF alpha in combination with the biotinylated anti-mouse/rat TNF alpha polyclonal antibody (13-7341) for detection and recombinant mouse TNF alpha (14-8321) or rat TNF alpha a
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, FC, N |
Regulatory Status | RUO |
Host | Hm |
Clone Number | [TN3-19.12] |
Genebank | TNF |
Isotype | IgG |
Crossreactivity | Ms, Rt |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.09% sodium azide |
Concentration | 0.5 mg/mL |
Working Dilution | Flow Cytometry (0.25 µg/test), ELISA (1-4 µg/mL), Neutralization (Assay-Dependent), Functional As... |
Purity | Affinity chromatography |
Conjugation | Unconjugated |
Storage Conditions | 4° C |
Disclaimer | Alternative CatNo: 14742385 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |