GFAP Monoclonal Antibody (GA5), eBioscience™

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Product Description

Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors. Applications Reported: This GA5 antibody has been reported for use in immunocytochemistry, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and flow cytometric analysis. Applications Tested: This GA5 antibody has been tested by immunoblotting of lysate prepared from mouse brain, immunocytochemistry of fixed and permeabilized C6 cells, and immunohistochemistry of FFPE human tissue