Syrian Hamster IgG Isotype Control, PE-Cyanine5, eBioscience™

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Product Description

Description: This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian hamster serum. Applications Reported: This isotype control antibody has been reported for use in flow cytometric analysis. Applications Tested: This isotype control antibody has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as the experimental antibody. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells;