CD80 (B7-1) Monoclonal Antibody (16-10A1), Functional Grade, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
16-0801-82 | 100 µg | 230,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis. 16-10A1 has also been reported in blocking of CD80 to its ligands. Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PA
Category | Antibody |
Supplier | ThermoFisher |
Application | FC, N |
Regulatory Status | RUO |
Host | Hm |
Clone Number | [16-10A1] |
Genebank | CD80 |
Isotype | IgG |
Crossreactivity | Dg, Ms, Pg |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with no preservative |
Concentration | 1 mg/mL |
Working Dilution | Neutralization (Assay-Dependent), Flow Cytometry (0.5 µg/test), Functional Assay (Assay-Dependent) |
Purity | Affinity chromatography |
Storage Conditions | 4° C |
Disclaimer | Alternative CatNo: 16080182 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |