CD85d (ILT4) Monoclonal Antibody (27D6), Functional Grade, eBioscience™

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Product Description

Description: The monoclonal antibody 27D6 recognizes the CD85d protein also known as ILT4, LIR-2. It is a member of the ILT (immunoglobulin-like transcript)/ LIR (leuokocyte Ig-like receptor) MIR (monocytes Ig-like receptor) family. CD85d is a 110 kDa single transmembrane receptor containing 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is confined to monocytes, macrophages and dendritic cells. The ligand for CD85d is non-classical class I HLA-G, -B and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM. Interaction of HLA-G with ILT4/CD85d is thought to contribute to tolerance during fetal implantation. Additionally it is the levels of ILT3 and ILT4 on DCs that suggests a role in organ allograft rejection. The monoclonal antibody 27D6 has been shown to block binding of ligand (HLA-G tetramer). Applications Reported: This 27D6 antibody has been reported for use in vitro in blocking assays and in flow cytometric analysis. Applications Tested: This 27D6 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will st