IL-1 beta Monoclonal Antibody (B122), Functional Grade, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
16-7012-38 | 5 mg | 3'280,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The B122 antibody reacts with mouse and rat interleukin-1beta (IL-1beta), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. Applications Reported: The B122 antibody has been reported for use in cytokine neutralization and ELISA capture. Applications Tested: The Functional Grade Purified B122 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-1 beta bioactivity. The B122 antibody at 0.125 µg/mL has been found to inhibit by 50% the biological effects of 4.0 ng/mL mouse IL-1 beta in a D10 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. The B122 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 beta (IL-1 beta) in combination with the biotin anti-mouse Interleukin-1 beta (IL-1 beta) polyclonal (13-7112) antibody for detection and recombinant mouse IL-1 beta (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve co
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, N |
Regulatory Status | RUO |
Host | Hm |
Clone Number | [B122] |
Genebank | IL1B |
Isotype | IgG |
Crossreactivity | Ms, Rt |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with no preservative |
Concentration | 1 mg/mL |
Working Dilution | Functional Assay (Assay-Dependent), Neutralization (Assay-Dependent), ELISA (0.125 µg/mL) |
Purity | Affinity chromatography |
Storage Conditions | 4° C |
Disclaimer | Alternative CatNo: 16701238 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |