Syrian Hamster IgG Isotype Control, APC, eBioscience™

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Product Description

Description: This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian hamster serum. Applications Reported: Golden Syrian hamster IgG isotype control has been reported for use in surface and intracellular flow cytometric analysis. Applications Tested: This Golden Syrian hamster IgG isotype control has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in other applications. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal