Sheep IgG Isotype Control

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Product Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Purity/Antibody specificity: Based on immunoelectrophoresis at an antigen concentration of 20 mg/mL, the pattern of precipitation against rabbit anti-sheep whole serum is the same as that against rabbit anti-sheep IgG, Fc fragment specific. This antibody is recommended for use in blocking assays, immunohistochemical, immunocytochemical and immunofluorescent applications. Store product at 4°C until opened. After opening, dilute only enough antibody for a single day's use. Store remainder at 4°C under sterile conditions. Country of Origin: USA The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin i