Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Rhodamine

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Product Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31685 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31685 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Rhodamine Amax= 550 nm; Emax= 570 nm. Fluorophore/Protein: 0.56 moles Rhodamine per mole IgG. (lot-dependent). Reconstitute with 0.4 mL of distilled water (1.5 mg/mL after restoration). Country of Origin: USA Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of