Rat IgG Isotype Control

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Product Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Purity: Based on immunoelectrophoresis at an antigen concentration of 20 mg/mL, the pattern of precipitation against goat anti-rat whole serum is the same as that against goat anti-rat IgG, Fc fragment specific. No precipitin line was detected against goat anti-rat IgA, alpha chain specific or goat anti-rat IgM, µ chain specific. This antibody is recommended for use in blocking assays, immunohistochemical, immunocytochemical and immunofluorescent applications. Storage: Store at 2-8ºC under sterile conditions. Dilute only enough antibody for a single day’s use. Country of Origin: USA The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful