F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, APC

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Product Description

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product # 31984 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product # 31984 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse, and rat serum proteins. However, this antibody may cross-react with immunoglobulins from other species. Phycobiliprotein Concentration: 0.5 mg/mL (determined by absorption = 73 at 650 nm for a 1% solution for only those APC molecules to which at least one molecule of active antibody is bound) Allophycocyanin Source: Spirulina platensis Store product protected from light at 4°C until opened. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. Do not freeze. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Allophycocyanin Amax= 650 nm; Emax= 660 nm. Reconstitute with 0.5 mL of distilled water (0.5