Claudin 2 Monoclonal Antibody (12H12)

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Product Description

This antibody reacts specifically with the ~22-23 kDa Claudin-2 protein. Reactivity has been confirmed with human, mouse, rat, and dog liver and kidney homogenates, as well MDCK and Caco-2 cell lysates, by western blotting and immunfluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon, spleen, and thyroid, and colon and breast cancer tissues by immunohistochemistry. For best results in immunohistochemistry (1-2 µg/mL) with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with EDTA buffer, pH 8.0, is required prior to staining. In western blotting a non-specific band at 27 kDa is observed in some lysates. In Caco-2 cells this band appears stronger than the correct band at 22 kDa. An alternative product, rabbit anti-Claudin-2 (Cat. No. 516100) may be used for western blotting of these lysates. Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell. TJ strands on adjacent cells are presumed to interact with