GAL4 Monoclonal Antibody
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
338600 | 200 µg | 466,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
33-8600 detects the Gal4 DNA Binding Domain by western blot, immunoprecipitation and immunofluorescence corresponding to amino acids 1-147 of the GAL4 DNA binding region. GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a "zinc finger" sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, ICC, WB |
Regulatory Status | RUO |
Immunogen | A recombinant protein corresponding to amino acids 1-147 mapping within the amino terminal, DNA binding domain of GAL4. |
Host | Ms |
Genebank | GAL4 |
Isotype | IgG1,k |
Crossreactivity | Yeast |
Clonality | Mab |
Format | Liquid; PBS; pH 7.4, with 0.1% sodium azide |
Concentration | 0.5 mg/mL |
Working Dilution | Immunocytochemistry (1:50 - 1:100), Western Blot (Assay-dependent), ELISA (Assay-dependent) |
Purity | Protein A |
Conjugation | Unconjugated |
Storage Conditions | -20°C |
Disclaimer | Alternative CatNo: 33-8600 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |