ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 488
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
339188 | 100 µg | 680,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This monoclonal antibody detects ZO-1alpha+ and ZO-1alpha- isoforms. The antibody is specific for ZO-1 proteins; cross-reactivity with the related ZO-2 protein has not been observed. Species reactivity includes human (Caco-2 Cell line) and dog (MDCK cell line). Based on sequence homology, reactivity with other species is likely but has not been confirmed. For Alexa Fluor 488 excitation 495 nm/emission 519 nm. Each lot of this antibody is tested for specificity by immunofluorescence. Expires one year from date of receipt when stored as instructed. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, ICC, WB |
Regulatory Status | RUO |
Immunogen | Human recombinant ZO-1 fusion protein encompassing amino acids 334-634 |
Host | Ms |
Clone Number | [ZO1-1A12] |
Genebank | TJP1 |
Isotype | IgG1 |
Crossreactivity | Dg, Hu |
Clonality | Mab |
Format | Liquid; PBS; pH 7.4, with 4mg/mL BSA and 0.1% sodium azide |
Concentration | 0.5 mg/mL |
Working Dilution | Western Blot (1-2 µg/mL), ELISA (0.1-1.0 µg/mL (unconjugated)), Immunocytochemistry (5 µg/mL) |
Purity | Protein A |
Conjugation | Alexa Fluor 488 |
Storage Conditions | 4° C, store in dark |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |