SR Monoclonal Antibody (16H3 (16H3E8))
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
339300 | 100 µg | 600,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
This monoclonal antibody can be used to detect a sub-set of the non-snRNP splicing factors termed SR proteins. The antibody detects SRp75, SRp55, SRp40, and SRp20 but not SRp30a or b (ASF/SF2, SC-35) proteins. 16H3 also recognizes approximately 20 distinct nuclear proteins including the U1 70K component of the U1 snRNP and both subunits of U2AF. The epitope detected by the 16H3 monoclonal antibody has been termed the “alternating arginine domain” and is composed almost exclusively of argnine alternating with glutamate and aspartate. 16H3 targets are predominantly nuclear, nonnucleolar, and are localized to active sites of polymerase II transcription. SR proteins are a highly conserved family of arginine/serine-rich, spliceosome-associated phosphoproteins essential for metazoan pre-mRNA splicing. SR proteins act early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also play a regulatory role because they can determine alternative splice site usage in vivo and in vitro. SR proteins appear to be recruited from nuclear “speckles”, in which they are concentrated to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus.
Category | Antibody |
Supplier | ThermoFisher |
Application | EL, ICC, WB |
Regulatory Status | RUO |
Immunogen | Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST. The 16H3 epitope has been mapped to a 40-amino acid polypeptide composed almost exclusively of arginine alternating with glutamate a... |
Host | Ms |
Clone Number | [16H3 (16H3E8)] |
Genebank | SRSF3 |
Isotype | IgG1,k |
Crossreactivity | Bo, Dg, Ck, Drosophila, Hu, Ms, Rb, Rt, Xenopus |
Clonality | Mab |
Format | Liquid; PBS; pH 7.4, with 0.1% sodium azide |
Concentration | 0.5 mg/mL |
Working Dilution | ELISA (0.1-1 µg/mL), Western Blot (3-5 µg/mL), Immunocytochemistry (0.5-2 µg/mL) |
Purity | Protein A |
Conjugation | Unconjugated |
Storage Conditions | Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C |
Disclaimer | Alternative CatNo: 33-9300 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |