CD25 Monoclonal Antibody (BC96), PE-Cyanine5.5, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
35-0259-42 | 100 Tests | 354,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation. Applications Reported: This BC96 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Ms |
Clone Number | [BC96] |
Genebank | IL2RA |
Isotype | IgG1,k |
Crossreactivity | Hu |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with 0.2% BSA and 0.09% sodium azide |
Concentration | 5 µL/Test |
Working Dilution | Flow Cytometry (5 µL (0.5 µg)/test) |
Purity | Affinity chromatography |
Conjugation | PE/Cy5.5 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 35025942 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |