Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine5.5, eBioscience™

* CHF, excl. 8.1% VAT and shipping costs

Product Description

Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: PE-Cy5.5 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis. Applications Tested: Rat IgG2b K Isotype Control PE-Cy5.5 has been tested by flow cytometric analysis of mouse splenocytes and human peripheral blood cells. This should be used at the same concentration as the experimental antibody. Excitation: 488-561 nm; Emission: 695 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary ant