CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Brilliant Violet™ 421, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
404-0621-80 | 25 µg | 140,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues. Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Brilliant Violet™ 421 (BV421) is a dye that emits at 423 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this f
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Rt |
Clone Number | [MEL-14] |
Genebank | SELL |
Isotype | IgG2a,k |
Crossreactivity | Ms |
Clonality | Mab |
Format | liquid; PBS; pH 7.2, with BSA and 0.09% sodium azide |
Concentration | 0.2 mg/mL |
Working Dilution | Flow Cytometry (0.125 µg/test) |
Purity | Affinity chromatography |
Conjugation | Brilliant Violet 421 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 404062180 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |