CD127 Monoclonal Antibody (A7R34), Brilliant Violet™ 421, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
404-1271-80 | 25 µg | 147,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127. Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis. Applications Tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Brilliant Violet™ 421 (BV421) is a dye that emits at 423 nm and is
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Rt |
Clone Number | [A7R34] |
Genebank | IL7R |
Isotype | IgG2a,k |
Crossreactivity | Ms |
Clonality | Mab |
Format | liquid; PBS; pH 7.2, with BSA and 0.09% sodium azide |
Concentration | 0.2 mg/mL |
Working Dilution | Flow Cytometry (1.0 µg/test) |
Purity | Affinity chromatography |
Conjugation | Brilliant Violet 421 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 404127180 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |