IDO Monoclonal Antibody (eyedio), Brilliant Violet™ 421, eBioscience™

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Product Description

Description: This eyedio monoclonal antibody reacts with human indoleamine-2,3-dioxygenase (IDO, INDO, IDO1), an intracellular enzyme that catalyzes the degradation of tryptophan to kynurenines. IDO is expressed in a wide variety of tissues and cells, including macrophages, plasmacytoid dendritic cells, and several cell lines. IDO can be induced in many different cell types by IFN gamma or other inflammatory stimuli. Expression of IDO in antigen presenting cells and tumors is thought to mediate immune suppression through depletion of this essential amino acid and/or by the creation of tryptophan metabolites that cause apoptosis of T cells and induction of regulatory T cells. Applications Reported: This eyedio antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eyedio antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at Category Antibody Supplier ThermoFisher Application FC Regulatory Status RUO Host Ms Clone Number [eyedio] Genebank IDO1 Isotype IgG1,k Crossreactivity Hu Clonality Mab Format liquid; PBS; pH 7.2, with BSA and 0.09% sodium azide Concentration 5 µL/Test Working Dilution Flow Cytometry (5 µL (0.125 µg)/test) Purity Affinity chromatography Conjugation Brilliant Violet 421 Storage Conditions 4° C, store in dark, DO NOT FREEZE! Disclaimer Alternative CatNo: 404947742 Tech. Data Sheet View datasheet Link To Supplier https://www.thermofisher.com Material Safety Data Sheet Download PDF