MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Brilliant Violet™ 711, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
407-5321-82 | 100 µg | 344,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes. Applications Reported: This M5/114.15.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Brilliant Violet™ 711 (BV711) is a tandem dye that emits at 713 nm an
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Rt |
Clone Number | [M5/114.15.2] |
Genebank | H2-AB1 |
Isotype | IgG2b,k |
Crossreactivity | Ms |
Clonality | Mab |
Format | liquid; PBS; pH 7.2, with BSA and 0.09% sodium azide |
Concentration | 0.2 mg/mL |
Working Dilution | Flow Cytometry (0.25 µg/test) |
Purity | Affinity chromatography |
Conjugation | Brilliant Violet 711 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 407532182 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |