Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Brilliant Violet™ 711, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
407-5981-82 | 100 µg | 326,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset. Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Rt |
Clone Number | [D7] |
Genebank | LY6A |
Isotype | IgG2a,k |
Crossreactivity | Ms |
Clonality | Mab |
Format | liquid; PBS; pH 7.2, with BSA and 0.09% sodium azide |
Concentration | 0.2 mg/mL |
Working Dilution | Flow Cytometry (1.0 µg/test) |
Purity | Affinity chromatography |
Conjugation | Brilliant Violet 711 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 407598182 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |