Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 570, eBioscience™

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Product Description

Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin. Applications Reported: This eBR2a antibody has been reported for use in immunohistochemical and immunocytochemical staining. Applications Tested: This Rat IgG2a Isotype Control eFluor® 570 has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells and by immunohistochemistry (IHC-P) on formalin-fixed paraffin embedded human tissue. Use isotype control at the same concentration as experimental antibody. Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable. Excitation: 555 nm; Emission: 570 nm The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant