CD3 Monoclonal Antibody (UCHT1), Brilliant Violet™ 650, eBioscience™
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
416-0038-41 | 25 Tests | 140,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Applications Reported: This UCHT1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UCHT1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Brilliant Violet™ 650 (BV650) is a tandem dye that emits at 649 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™,
Category | Antibody |
Supplier | ThermoFisher |
Application | FC |
Regulatory Status | RUO |
Host | Ms |
Clone Number | [UCHT1] |
Genebank | CD247 |
Isotype | IgG1,k |
Crossreactivity | Hu |
Clonality | Mab |
Format | Liquid; PBS; pH 7.2, with BSA and 0.09% sodium azide |
Concentration | 5 µL/Test |
Working Dilution | Flow Cytometry (5 µL (0.5 µg)/test) |
Purity | Affinity chromatography |
Conjugation | Brilliant Violet 650 |
Storage Conditions | 4° C, store in dark, DO NOT FREEZE! |
Disclaimer | Alternative CatNo: 416003841 |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.thermofisher.com |
Material Safety Data Sheet | Download PDF |