TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), Brilliant Violet™ 650, eBioscience™

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Product Description

Description: The GL3 monoclonal antibody reacts with the gamma delta T-cell Receptor complex (TCR) on all gamma delta TCR bearing T cells, but it does not react with alpha beta TCR. The gamma delta TCR is present on cells in the thymus, epidermis, epithelial lining of the intestine, peritoneal cavity, and lymphoid tissues. It is recommended to prestain cells with CD16/CD32 Monoclonal Antibody (Product # 14-0161-82) to prevent non-specific Fc-mediated binding of the GL3 monoclonal antibody. Applications Reported: This eBioGL3 (GL-3, GL3) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioGL3 (GL-3, GL3) antibody has been tested by flow cytometric analysis of mouse lymph node cells. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Brilliant Violet™ 650 (BV650) is a tandem dye that emits at 649 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrum