Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 615, eBioscience™

* CHF, excl. 8.1% VAT and shipping costs

Product Description

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin. Applications Reported: This eBRG1 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded and frozen tissue sections and immunocytochemistry. Applications Tested: This eBRG1 antibody has been tested by immunocytochemistry on fixed and permeabilized cells. Isotype control should be used at the same concentration as experimental antibody. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered. The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific backg